Localization of Cytochrome b-559 in the Chloroplast Thylakoid Membranes in Spinach.
نویسندگان
چکیده
Cytochrome b-559 was purified from spinach leaves and antibodies were made against it in rabbit. Using affinity-purified, monospecific antibodies, we have found that cytochrome b-559, which is closely associated with the primary photochemical activity of photosystem II, is localized exclusively in the grana thylakoids.
منابع مشابه
Synthesis of thylakoid membrane proteins by chloroplasts isolated from spinach. Cytochrome b559 and P700-chlorophyll a-protein
Intact chloroplasts, purified from spinach leaves by sedimentation in density gradients of colloidal silica, incorporate labeled amino acids into at least 16 different polypeptides of the thylakoid membranes, using light as the only source of energy. The thylakoid products of chloroplast translation were visualized by subjecting membranes purified from chloroplasts labeled with [35S]methionine ...
متن کاملIsolation and Some Molecular Properties of Plastidic Algal Cytochrome b-559
Cytochrome b-559, an integral protein of diloroplast thylakoids, was prepared in a homogeneous form from the alga Bum illeriopsis filiform is. The protein is easily denatured and can be solubilized by treatment of isolated thylakoids with high concentrations of urea and detergents in addition to weak sonification. The reduced form exhibits absorption maxima at 559, 530 and 429 nm. By comparativ...
متن کاملMembrane rupture is the common cause of damage to chloroplast membranes in leaves injured by freezing or excessive wilting.
The effects of freezing and desiccation of spinach leaves (Spinacia oleracea L. cv Yates) on the thylakoid membranes were assessed using antibodies specific for thylakoid membrane proteins. The peripheral part of the chloroplast coupling factor ATPase (CF1) was used as a molecular marker for chemical membrane damage by chaotropic solutes. Plastocyanin, a soluble protein localized inside the clo...
متن کاملFerredoxin:NADP+ oxidoreductase is a subunit of the chloroplast cytochrome b6f complex.
Purified detergent-soluble cytochrome b6f complex from chloroplast thylakoid membranes (spinach) and cyanobacteria (Mastigocladus laminosus) was highly active, transferring 300-350 electrons per cyt f/s. Visible absorbance spectra showed a red shift of the cytochrome f alpha-band and the Qy chlorophyll a band in the cyanobacterial complex and an absorbance band in the flavin 450-480-nm region o...
متن کاملDithiol oxidant and disulfide reductant dynamically regulate the phosphorylation of light-harvesting complex II proteins in thylakoid membranes.
Light-induced phosphorylation of light-harvesting chlorophyll a/b complex II (LHCII) proteins in plant thylakoid membranes requires an activation of the LHCII kinase via binding of plastoquinol to cytochrome b(6)f complex. However, a gradual down-regulation of LHCII protein phosphorylation occurs in higher plant leaves in vivo with increasing light intensity. This inhibition is likely to be med...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Plant physiology
دوره 80 1 شماره
صفحات -
تاریخ انتشار 1986